Page 1 bd facscanto ii flow cytometer quick reference guide this guide contains instructions for both bd facscanto clinical and bd facsdiva software. Bd facscanto ii is a flow cytometer intended for the qualitative and quantitative measurement of. Bd horizon brilliant polymer dyes are four to ten times brighter than conventional dyes, with equivalent backgrounda breakthrough in the field. The ivd cleared bd facscanto ii system includes software designed to address the needs of todays busy clinical laboratory. Chetna dwivedi clinical system verification engineer ii. It provides a high degree of automation and quality control to reduce hands on time for technicians and improve. Analysis of flow cytometry patient data of clinical trials using bd facsdiva software. If you are not familiar with the windows operating system, refer to the documentation provided with your computer. Flow cytometry analyser ivd, automated a mains electricity acpowered laboratory instrument intended to be used to count, examine andor sort cells or microscopic particles in a clinical specimen e.
It automatically sets cytometer detector voltages and creates compensation tables based on a single run with bd. The bd facscanto ii system delivers flow cytometry data on an efficient, user friendly platform. Discover clinical simplicity with the bd facsvia flow cytometry system. Bd biosciences facscanto ii flow cytometer product list. Using bd panels with bd facs spa and bd facscanto clinical software and the bd facslink lis interface. Use bd facscanto clinical software for performing cytometer qualitycontrol. The development of seven and eightcolor assays on this device will require. Collating data from global sites, data managing and uploading to the sponsor sites as per. Bd facsdiva software efficiently controls the setup, acquisition, and analysis of flow cytometry data from the bd facscanto ii workstation. System options include 8color 3laser system 405 488 633 nm 8color 2laser system 488 633 nm a 2d.
Minimize, maximize, and close buttons in title bar minimize buttonreduces the application to a button on the windows taskbar. Our bd facscanto ii is a 3 laser red 633nm, blue 488nm and violet 405nm cytometer capable of analyzing up to 8 fluorescence parameters and 2 scatter parameters fsc and ssc. Instrument optimization for bd facscanto instruments creating application settings for white blood cells using lysewash, or lyseno wash methods. Bd biosciences technical field service engineer can be. Stratedigm offers its flow cytometers as a superior alternative to the bd lsr ii and bd fortessa. Jul 05, 20 facscalibur ii is a stalwart workhorse in the clinical and research lab with a 3 color, 1 laser blue system, or 4 color 2 laser blue, red system. The bd facscanto ii system can optionally be used with the bd facs loader for automatic sample introduction, a standalone barcode reader for data input into bd facscanto clinical software, and with the bd facs sample prep assistant ii. Contact us today at 408 5123901 for further details. Bd facsdiva software reference manual for version 8.
Cytometry bd facscanto ii laser errors happy new year to all. It contains important information that is not included in the printed or electronic documentation. Win32k patch was released with december 2019 security updates, crypto api patch. Built on more than 30 years of bd experience and leadership in flow cytometry and multicolor analysis, the bd facscanto flow cytometry system delivers reliable performance, accuracy, and easeofuse for todays busy clinical laboratories. Saves time, improves reproducibility high performance, innovative system the bd facscanto ii fluidics cart is. Bd synapsys solution allows connectivity for any number of supported instruments to one or more lis systems. A fixed alignment flow cell, for example, minimizes startup time and improves reproducibility. Bd bioscience facscanto ii flow cytometer marshall. Figure 11 bd facscanto ii flow cytometer the bd facscanto ii flow. Updated for 6color reagent and bd facscanto clinical software v2. Bd facs canto ii operators manual marshall scientific. Bd facscanto ii canto 10color with canto clinical software. Samples were analyzed on the bd facslyric system with bd facsuite clinical software, and on the bd facscanto ii system with bd facscanto clinical software and bd facs 7color. Bd facscanto clinical software overview features workflow sample data productivity, consistency, and reliability from dedicated clinical software the ivd cleared bd facscanto ii system includes software designed to address the needs of todays busy clinical.
Collating data from global sites, data managing and. The bd facscanto ii provides the ultimate in flexibility and can be configured with two or three. Bd biosciences bd facscanto ii flow cytometers 422 and 53 configurations with bd facscanto clinical software and bd facsdiva software. Bd facscanto clinical software is supplied with the bd facscanto and facscanto ii systems and includes application panels optimized for use with specific ivd reagent kits. Firsttime users of bd facsdiva software should read.
The innovative designs for both the excitation and collection optics reduce excitation losses, yielding more information from each sample. This software is for use only on the bd facscanto and bd facscanto ii. Multisite evaluation of the bd stem cell enumeration sce. Bd facsdiva software provides a wide array of basic analysis features, enabling multiple applications to run on the bd facscanto, bd lsr, and bd facsaria cytometers. Find a wide selection of bd biosciences facscanto systems in auctions. Bd facscanto ii becton dickinson, san jose, ca, usa data file structure. It includes automated setup and compensation, applicationspecific analysis modules, and predefined reports with integrated quality control. The february 2020 security rollupcumulative update was released in february 2020. Bd facscanto ii flow cytometer quick reference guide this guide contains instructions for both bd facscanto clinical and bd facsdiva software. Bd facsdiva operating software is common across many bd cell analyzers and cell sorters, including bd lsr and bd. Principles of flow cytometryprinciples of flow cytometry mohamed labib salem, phd prof.
These tools assist in the ease and efficiency of using facscanto as they enable automated features. Bd biosciences facscanto ii flow cytometer features optics. Bd facscanto fluidics with bd facscanto clinical software or bd facsdiva software. Bd facscanto clinical software set up the cytometer with bd facscanto clinical software. Bd facs canto ii flow cytometry system gmi trusted. The bd facscanto ii is a powerful multicolour benchtop analyser offering easy to use, powerful and reliable performance. Apart from the diva software, the bd facscanto ii system includes a clinical software designed to address the needs of todays busy clinical laboratory. Bd facsdiva software for setup, acquisition, and analysis bd facsdiva software efficiently controls the setup, acquisition, and analysis of flow cytometry data from the bd facscanto ii workstation. Bd facscanto ii flow cytometer reference manual alfresco. Jan 21, 2012 facscanto ii offers walkaway sample handling with the carousel loader 40 tubes capacity or high throughput sampler 96 or 384well plate loader. A proven platform for high reliability and quality results.
Standardization of 8color flow cytometry across different. Becton dickinson bd facscanto ii and beckman coulter bc navios, aiming at increasing reproducibility and interlaboratory comparability of immunophenotypic data in clinical. The pleurx system helps patients take control of uncomfortable and painful symptoms from pleural effusions or malignant ascites. Bd facscanto ii becton dickinson, san jose, ca, usa. Bd facscanto ii quick reference manual pdf download. It delivers productivity, consistency, and reliability with features that include automated setup and compensation using bd facs.
Win32k patch was released with december 2019 security updates, crypto api patch was released with january 2020 security updates. Home products manufacturers bd biosciences bd facscanto ii products. Built on more than 30 years of bd experience and leadership in flow cytometry and multicolor analysis, the bd facscanto flow cytometry system delivers reliable performance, accuracy, and easeofuse for todays busy clinical. The optics of the bd facscanto ii system consist of an. It delivers productivity, consistency, and reliability with features that include automated setup and compensation using bd. Manage symptoms from pleural effusions or malignant ascites at home. Standardization of 8color flow cytometry across different flow cytometer instruments. Pdf accurate and reproducible enumeration of t, b, and nk. Accurate and reproducible enumeration of t, b, and nk. New worksheet features create headers and footers on the worksheet, including cytometer name, experiment name, user name, date and time, bd facsdiva software version, etc. Bd facs canto ii operators manual marshall scientific issuu. Bd synapsys solution provides secure and scalable connectivity for bd bactec blood culture solution, bd kiestra lab automation solution and, in the u. Running samples with bd facscanto clinical software. The bd facscanto family of benchtop analyzers features reliable performance and accurate results for experiments requiring up to 10 parameters.
Bd facscanto clinical software with spa ii sample prepper bd facscanto clinical software with spa iii sample prepper using bd panels with bd facscanto clinical software and the bd facslink lis interface. Bd biosciences facscanto ii flow cytometer features. Bd facsdiva software pc performs sample acquisition 18 bits5 decades and data analysis. Numerous innovations make the system easy to use, powerful, and productive. A june 2007 bd biosciences benex limited canada japan bay k 1 ad nippon becton. Multisite evaluation of the bd stem cell enumeration kit for. The expert staff at nyu langones cytometry and cell sorting laboratory provides researchers with training on and access to a facscanto analyzer.
Find out firsthand how the unique capabilities of the bd facsvia flow cytometry system are designed with clinical simplicity in mind for excellence in blood cell enumeration and analysis. View christopher sequeira ms, ccys profile on linkedin, the worlds largest professional community. Follow only those sections that apply to the software you are running. It delivers productivity, consistency, and reliability with features that include automated setup and compensation using bd facs 7color setup beads, applicationspecific analysis modules, and predefined reports with integrated quality control. Before you begin z stain either the high or the low bd stem cell control. Applications are far reaching and can be found on the bd online tutorial.
The software uses an automatic gating algorithm to identify and isolate stem cells. Sections are indicated with the appropriate software. A feasibility study in clinical laboratories in switzerland. Instrument installation, operational, and perf clinical cytometry.
At a time when health care systems around the globe are struggling to keep up with the influx of patients from the covid19 crisis, bd employees with clinical backgrounds are stepping up to help. A bd hlab27 software module for the bd facscanto ii system provides a complete system for rapid detection of hlab27 antigen, which is clinically relevant to the evaluation of seronegative spondyloarthropathies. Application name bd facsdiva, bd facscanto clinical software. Software acquisition and analysis methods are based on the method featured in the clinical. Bd facscanto clinical software further automates setup, compensation analysis, and quality control for predefined clinical applications. Bd biosciences recommends that firsttime users of this cytometer. As a leading provider of tools for flow cytometry, bd biosciences is committed to offering comprehensive, innovative software. One striking difference between the facscanto and older generation machines is that this system operates on a pcbased windows xp platform using bd facsdiva software. These panels feature automated gating, calculations, and report generation to deliver consistent, reproducible, and standardized analysis. Bd facscanto ii flow cytometer quick reference guide. The bd bioscience facscanto ii flow cytometer is a benchtop analyzer providing reliable and accurate results for experiments with up to 10 parameters.
Jun 07, 2006 the bd facscanto is often hailed as the new generation of flow cytometry. Bd facs sample prep assistant iii accessory to the bd facscanto system with bd facscanto clinical software. The bd facscanto ii system uses a fixedoptics design and advanced digital. Issuu is a digital publishing platform that makes it simple to publish magazines, catalogs, newspapers, books, and more online. The optional bd facscanto ii handheld barcode reader reads twodimensional barcode labels and it is used to read the compensation matrix of the bd facs 7color setup beads reagent into bd facscanto clinical software.
All other cytometer and fluidics cart functions are controlled by bd facscanto clinical software and bd facsdiva software provided with the instrument. Built on more than 25 years of bd experience and leadership in flow cytometry and multicolor analysis, the bd facscanto ii system is an easytouse benchtop analyzer that delivers proven performance, accuracy, and highquality results. Christopher sequeira ms, ccy technical training engineer. The bd facscanto ii is designed to address the needs of todays busy clinical lab. Its flexible, modular design offers automation and up to eightcolor capacity for rapid and easy production of highquality data in clinical. Bd biosciences recommends that firsttime users of this cytometer take advantage of operator training offered with. Daily routine procedures such as startup, shutdown, and cleaning routine cycles are automated as a result of fluidic integration with bd facscanto clinical software or bd facsdiva software. Bd biosciences recommends that firsttime users of this cytometer takeadvantage of. Setup on page 1 in the bd facsdiva software reference manual. Page 1 bd facscanto ii instructions for use for in vitro diagnostic use part no. Bd facscanto flow cytometer 433 configuration with bd facscanto clinical software and bd facsdiva software and bd facscanto ii flow. Cell protein dna core facility georgia state university.
The bd facscanto ii system can optionally be used with the bd facs loader for automatic sample introduction, a standalone barcode reader for data input into bd facscanto clinical software, and with the bd facs sample prep assistant ii for automatic sample preparation of assays utilizing the lysenowash method. Bd facs canto ii operators manual marshall scientific by. The facscanto ii offers unbeatable consistency and optimal signal detection with increased resolution and sensitivity for multicolor assays. The bd facscanto ii provides the ultimate in flexibility and can be configured with two or three lasers to detect up to eight colors. Flow cytometry patient data of clinical trials using bd facsdiva software. Bd bioscience facscanto ii flow cytometer marshall scientific. Provides streamlined software workflows for acquisition and analysis.